Dinucleotide repeat polymorphism at the human gene for insulin-like growth afctor I (IGFI)
نویسندگان
چکیده
منابع مشابه
Dinucleotide repeat polymorphism at the human preproglucagon gene.
References: 1) Bell.G.I. etal. (1983) Nature 304, 368-371. 2) Weber.J.L. and May.P.E. (1989) Am. J. Hum. Genet. 44, 3 8 8 3 % . 3) Schroeder.W.T. et al. (1984) Cytogenet. Cell Genet. 38, 76-79. 4) Weber,J.L. etal. (1990) Nucl. Acids Res. 18, 4637. Source/Description: The polymorphic (TG)n repeat begins at base pair 3833 in intron D of the human interleukin 9 gene (IL-9) on chromosome 5 (1). The...
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The aim of the present study was to detect polymorphism in Insulin like growth factor-I (IGF-I) gene of beluga (Huso huso) fish using PCR-SSCP technique and also investigation of its association with growth traits (condition factor, body length and weight). A total of 150 specimens of beluga were randomly selected and DNA was isolated from caudal fin using modified salting out method. Then two ...
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PCR Conditions: PCR was performed in a total volume of 20 microliters with 200 ng of genomic DNA, 8 pmoles of each primer, 2 mM MgCl2, 200 mM dNTP, 50 mM KC1, 5 mM Tris-HCl, pH 8.3, 0.01% gelatin, 0.5 U Taq polymerase (Boehringer). Primer Ang5 was 5' end labeled with P 7-ATP with the resulting specific activity 0.1-0.05 /iCi/pmol. Amplification was for 35 cycles with denaturation at 94°C 1 min,...
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References: 1) Oshima,A. et al. (1988) J. Biol. Chem. 263, 2553-2562. 2) Laureys.G. etal. (1988) Genomicsi, 224-229. 2/162 6/162 129/162 20/162 1/162 4/162 0.01 0.04 0.80 0.12 0.01 0.02 Source/Description: The clone VAW140 (1) was tested for CA repeats by Southern blot hybridization (2). One CA-hybridizing Hindm fragment was subcloned into mp8 in both orientations and sequenced at each end. A (...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.20.5797-a